She has been working on testicular physiology from the early eighties.
Since1995, she has been involved in the development of original male germ cell culture systems (BioAlteR®) in order to study ex vivo local regulation of spermatogenesis, in Philippe Durand's team (INSERM U418, IGFL at ENS Lyon).She is a specialist in the study of the regulations of spermatogenesis, in these cultures and of cytological study of these cells Since 2007, BioAlteR® is used for testicular toxicology.
She is co-founder of the company Kallistem, created in 2012, (http://www.kallistem.com/en/) from BioAlteR®. Kallistem offers services in predictive toxicology in the male reproductive field and meets the needs of the pharmaceutical and chemical industries.
She joined INSERM U1208 in 2013.
She has developed a new culture system ARTISTEM® which allows the whole ex vivo spermatogenesis in human, monkey, rat (world premiere) and mouse, in collaboration with Kallistem and the Institute of Polymer Materials. The aim of ARTISTEM® is to preserve and restore male fertility.
ARTISTEM® should also be of interest for the research of Pierre Savatier's team: indeed, it takes 4 years for transgenic newborn monkeys to reach adulthood and the ability to reproduce. The possibility of maturing spermatogonia of very young males ex vivo will make it possible to transmit the transgene to the offspring much more quickly (drastic decrease of generation intervals).
|2016||95(4):89||Complete Human and Rat Ex Vivo Spermatogenesis from Fresh or Frozen Testicular Tissue||Perrard MH, Sereni N, Schluth-Bolard C, Blondet A, D Estaing SG, Plotton I, Morel-Journel N, Lejeune H, David L, Durand P||Biol Reprod||-|
|2009||4(10):e7237||Cytostatic factor proteins are present in male meiotic cells and beta-nerve growth factor increases mos levels in rat late spermatocytes||Perrard MH, Chassaing E, Montillet G, Sabido O, Durand P||PLoS One||-|
|2017||45(Pt 3):366-373||Effects of low doses of carbendazim or iprodione either separately or in mixture on the pubertal rat seminiferous epithelium: An ex vivo study||Durand P, Martin G, Blondet A, Gilleron J, Carette D, Janczarski S, Christin E, Pointis G, Perrard MH||Toxicol In Vitro||-|
|2015||Volume 1, Number 4, 289-301||Endocrine Disrupting Effects of Noncytotoxic Doses of Carbendazim on the Pubertal Rat Seminiferous Epithelium: An Ex Vivo Study||Carette D, Blondet A, Martin G, Montillet G, Janczarski S, Christin E, Pointis G, Durand P, Perrard MH||Applied in Vitro Toxicology||-|
|2013||268(1):27-36||Hexavalent chromium at low concentration alters Sertoli cell barrier and connexin 43 gap junction but not claudin-11 and N-cadherin in the rat seminiferous tubule culture model||Perrard MH, Carette D, Prisant N, Gilleron J, Pointis G, Segretain D, Durand P||Toxicol Appl Pharmacol||-|
|2017||19 (4) : 279-89||La spermatogenèse ex-vivo||Perrard MH, Durand P||Médecine Thérapeuthique-Médecine de la Reproduction, numéro spécial "Spermatozoïde et spermatogenèse : données actuelles"||-|
|2016||60:92-103||Use of a rat ex-vivo testis culture method to assess toxicity of select known male reproductive toxicants||Goldstein KM, Seyler DE, Durand P, Perrard MH, Baker TK||Reprod Toxicol||-|