Pluripotent stem cells (ESC, EpiSC or iPSC) from different species are routinely cultured in vitro and represent a tremendous hope for numerous therapies. However, the derivation of ES cells and the production of iPS cells are very inefficient in non-rodent species; the quality of these cells remains variable; and only rodent ES and iPS cells maintain the most naive pluripotency state similar to the inner cell mass. Therefore, the objectives of my research are to improve the derivation and purification of safe pluripotent cells. For these purposes, my current projects focus on:
- understanding the mechanisms involved in the maintenance of naive or primed states by focusing on the retroviral silencing in mouse, as a model, in chicken and duck (non-mammalian) and rabbit (mammalian) as three non-rodent models.
- defining novel biomarkers to distinguish pluripotency states.
- developing new tools such as reporter genes to mark and/or select pluripotent stem cells.
- I participated in the establishment of reprogrammed avian somatic cells.
- I derived duck embryonic stem cells.
- I developed novel reporter genes which mark and enrich cell populations according to their pluripotency status.
Last significant publications:
Fuet A, Montillet G, Jean C, Aubel P, Kress C, Rival-Gervier S, Pain B, 2018. NANOG Is Required for the Long-Term Establishment of Avian Somatic Reprogrammed Cells. Stem Cell Reports, 11(5):1272-1286.
Chen YC, Chang WC, Lin SP, Minami M, Jean C, Hayashi H, Rival-Gervier S, Kanaki T, Wu SC, Pain B, 2018. Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003. PLoS One, 13(9):e0200515.
Pain B, Kress C, Rival-Gervier S, 2018. Pluripotency in avian species. Int J Dev Biol, 62(1-2-3):245-255.
Donega V, Marcy G, Lo Giudice Q, Zweifel S, Angonin D, Fiorelli R, Abrous DN, Rival-Gervier S, Koehl M, Jabaudon D, Raineteau O., 2018. Transcriptional Dysregulation in Postnatal Glutamatergic Progenitors Contributes to Closure of the Cortical Neurogenic Period. Cell Rep., 22(10):2567-2574.
Rival-Gervier S, Lo MYM, Khattak S, Pasceri P, Lorincz MC and Ellis J, 2013. Kinetics and Epigenetics of Retroviral Silencing in Mouse Embryonic Stem Cells Defined by Deletion of a D4Z4 Barrier Element. Molecular Therapy, 21 (8), 1536-1550.