Bertrand Pain Team Leader / DR2
Our work aims to decipher cellular and molecular mechanisms controlling the establishment and the maintenance of pluripotency in reference and agronomic species including porcine, bovine and equine but also Aves as models of non-mammalian species. By developing and studying these pluripotent stem cells (PSCs), we articulate our research project around two main axes.
Axis 1: Derivation and characterization of pluripotent stem cells (PSCs) in different agronomic species:
We were pioneers in the isolation, in vitro establishment and characterization of chicken embryonic stem cells (ESC) (Pain et al, 1996; Lavial et al., 2007, Pain et al., 2018). This long term establishment is achieved using various culture media, which leads to different pluripotent stem cells that are then characterized at the molecular, epigenetic and developmental levels (Jean et al., 2013; Kress et al., 2016). Recently, we also demonstrated that the somatic reprogramming, defined as the process to get induced pluripotent stem cells (iPSCs) is possible but partially successful in avian species (Fuet et al., 2018). In order to improve the derivation of pluripotent cells and the reprogramming, we develop reporter genes based on endogenous retroviral elements (ERV) to enrich cell populations according to their pluripotency status.
Primordial germ cells (PGC), which are closely related to ESCs, are specified differently in avian species compared to mammals. We also isolated avian PGCs, grew them in vitro in long term cultures and compared their transcriptomes (RNAseq) and epigenomes (ChIPseq) to the ESC ones. Advances in mastering large scale 3D culture of PGCs (Chen et al., 2018) as well as in establishing duck PGC culture system (Chen et al., 2019) allows us to compare these cultured germ cells with the ones found in the gonads.
Using either the canonical Yamanaka gene cocktail or new gene combinations (Baquerre et al., 2017, EP17305082.4, patent, Aurine et al., 2018) the somatic reprogramming approach was applied to various mammalian species including human, bovine, porcine, equine and bat derived cells. Those different reprogrammed cells were characterized by immunochemistry (cell markers) and biochemistry (alkaline phosphatase and telomerase activity) and compared with the starting primary cells as well as with the early embryos by RNAseq. All together those data provide a comparison basis for establishing the pluripotency networks in all those species with a phylogenetic perspective.
Axis2: Biotechnology of pluripotent cells:
PSCs are a unique source of cell plasticity to generate specific derivatives by controlling their differentiation process. By applying various protocols of differentiation to engage the pluripotent stem cells toward different lineages, a large spectrum of differentiated progenitors could be produced (Vautherot et al., 2013, N° FR1357346, patent, Couteaudier et al., 2015; Vautherot et al., 2017). Beside the fundamental research, for which obtaining and characterizing specific differentiated cells from pluripotent stem cells is a real challenge, one of the main interests of those pluripotent and iPS cells lies in their biotechnological potential. In particular, these cells and their differentiated derivatives are useful cellular substrates for viral and bacterial studies as well as vaccine production (Giothis et al., 2019)
|2015||6:7095||Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency||Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, Rognard C, Hochard A, Voisin S, Fontaine E, Mure M, Afanassieff M, Cleroux E, Guibert S, Chen J, Vallot C, Acloque H, Genthon C, Donnadieu C, De Vos J, Sanlaville D, Guérin JF, Weber M, Stanton LW, Rougeulle C, Pain B, Bourillot PY, Savatier P||Nat Commun||-|
|2015||14(2):224-37||Derivation of keratinocytes from chicken embryonic stem cells: establishment and characterization of differentiated proliferative cell populations||Couteaudier M, Trapp-Fragnet L, Auger N, Courvoisier K, Pain B, Denesvre C, Vautherot JF||Stem Cell Res||-|
|2015||14(1):54-67||Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC||Jean C, Oliveira NM, Intarapat S, Fuet A, Mazoyer C, De Almeida I, Trevers K, Boast S, Aubel P, Bertocchini F, Stern CD, Pain B||Stem Cell Res||-|
|2013||55(1):41-51||Pluripotent genes in avian stem cells||Jean C, Aubel P, Soleihavoup C, Bouhallier F, Voisin S, Lavial F, Pain B||Dev Growth Differ||-|