User :       
Password :
      Not yet a member ?


Fifth French Conference on Computation Neurosciences: Neurocomp10

Published on March 5, 2010

Lyon is hosting the fifth French Conference on Computational Neurosciences...
read more


 

 

 Teams 

Reproduction, epigenesis and development

 

One of the conducting thread that permits evolution from the oocyte up to embryonic stem (ES) cells is epigenetic remodeling, particularly via the parental imprinting process.
Imprints establishment occurs during gametogenesis and is maintained in somatic cells through embryonic development and post-natal life.
Our team examines the role of different perturbators on genomic imprinting ressetting during gametogenesis and on genomic imprinting expression in stem cells.
Part of the team is involved in developping new processes to preserve women fertility. This includes ovary cryopreservation and grafting and ovocytes vitrification that are validated according to the integrity of genomic imprints.
The other part of the team will evaluate the stability of the imprint in stem cell lines in collaboration with C Dehay and P Savatier Teams



Annick Elisabeth Lefèvre

Genomic imprinting in oocytes and stem cells


Mohamed Al Khtib PhD student

- Evaluation of the  human immature oocyte vitrification, followed by IVM, on the methylation profile of H19 and KCNQ1OT1 imprinting centres.

 

- Methylation of the pluripotency factor promoters  in human embryos at 2-4 cell   stage, progressive or blocked in their development. 

 

My CV in french :

http://m-alkhtib.cv.fm/fr/


Thierry Blachere

Molecular biology, DNA methylation, imprinted genes.


Alexis Forterre

Analysis of the methylation profile of 2 imprinted loci (Igf2/H19 and  Snrpn)in primate embryonic stem cells


Rita Khoueiry PHD student

Differential methylation of KCNQ1OT1 in  human oocytes and embryos. Evaluation of the impact of hormonal stimulation and cell culture.


Jacqueline Lornage

Cryopreservation of ovaries and oocytes development


Claire Mazoyer

Cryobiology, histology and follicular development


Myriam Momier PHD student

Subject: Vitrification of whole sheep ovary

I work on whole sheep ovary cryopreservation through vitrification. The purpose is to conserve the integrity and functionality of the ovary at very low temperature. The aim of this study is to validate a technic to preserve woman fertility prior to cancer tratement. Cryopreserved ovaries will be grafted to the patients following cure.


Bruno Salle

Ovary autograf and development


Delphine Vertu-Ciolono

Ovary graphting


Selected Publications of Team members :
Borghol N, Blachere T, Lefevre A. (2008) Transcriptional and epigenetic status of protamine 1 and 2 genes following round spermatids injection into mouse oocytes Genomics 91 (5): 415-422 download
Courbiere B, Caquant L, Mazoyer C, Franck M, Lornage J, Salle B. (2008) Difficulties improving ovarian functional recovery by microvascular transplantation and whole ovary vitrification Fertil Steril download
Khoureiry R, Ibala-Rhomdane S, Mery L, Blachere T, Guerin JF, Lornage J, Lefevre A. (2008) Dynamic CpG methylation of the KCNQ1OT1 gene during maturation of human oocytes J Med Genet 45 (9): 583-8 download
Baudot A, Courbiere B, Odagescu V, Salle B, Mazoyer C, Massardier J, Lornage J. (2007) Towards whole sheep ovary cryopreservation Cryobiology 55 (3) : 236-48 download
Borghol, N. Lornage, J. Blachere, T. Sophie Garret, A. Lefevre, A. (2006) Epigenetic status of the H19 locus in human oocytes following in vitro maturation Genomics 87 (3): 417 - 426 download
Courbiere B, Odagescu V, Baudot A, Massardier J, Mazoyer C, Salle B, Lornage J. (2006) Cryopreservation of the ovary by vitrification as an alternative to slow-cooling protocols Fertil Steril 86 (4 suppl) download
Bordes A, Lornage J, Demirci B, Franck M, Courbiere B, Guerin JF, Salle B. (2005) Normal gestations and live births after orthotopic autograft of vitrified-warmed hemi-ovaries into ewes Hum Reprod 20 (10): 2745-8 download
Courbiere B, Massardier J, Salle B, Mazoyer C, Guerin JF, Lornage J. (2005) Follicular viability and histological assessment after cryopreservation of whole sheep ovaries with vascular pedicle by vitrification Fertil Steril 84 (suppl 2) 1065-71 download
Salle B, Demirci B, Franck M, Berthollet C, Lornage J. (2003) Long-term follow-up of cryopreserved hemi-ovary autografts in ewes: pregnancies, births, and histologic assessment Fertil Steril 80 (1): 172-7 download
Salle, B. Demirci, B. Franck, M. Rudigoz, R. C. Guerin, J. F. Lornage, J. (2002) Normal pregnancies and live births after autograft of frozen-thawed hemi-ovaries into ewes Fertil Steril 77 (2): 403-8 download
Ziyyat A, Lefevre A (2001) Differential gene expression in pre-implantation embryos from mouse oocytes injected with round spermatids or spermatozoa Hum Reprod 16 (7): 1449-56 download
Salle B, Lornage J, Demirci B, Vaudoyer F, Poirel MT, Franck M, Rudigoz RC, Guerin JF (1999) Restoration of ovarian steroid secretion and histologic assessment after freezing, thawing, and autograft of a hemi-ovary in sheep Fertil Steril 72 (2): 366-70 download
Salle B, Lornage J, Franck M, Isoard L, Rudigoz RC, Guerin JF (1998) Freezing, thawing, and autograft of ovarian fragments in sheep: preliminary experiments and histologic assessment Fertil Steril 70 (1):124-8 download