Physiology and biotechnology of avian stem cells
The team is focusing its research on two major questions related to the physiology and the biotechnology use of avian embryonic stem cells:
| Bertrand Pain || |
Interest in investigating the ES cells physiology. Our research group aims to understand the molecular mechanisms that control pluripotency, somatic and germinal differentiation using the chicken as a non mammalian model system.
Interest in compairing stem and germ cell physiology in different avian and mammalian species of agronomical interest.
Interest in developing new cell lines from various species as substrates for viral vaccine replication.
| Camille Baquerre || |
| Christian Jean || |
Chicken gene analysis by qPCR and Microarray.
| Clémence Kress ||
My research focuses on how epigenetic mechanisms control the pluripotency and the differentiation of avian embryonic stem cells. I aim to characterize the dynamics of DNA methylation and chromatin modifications in cultured cells and during the early development of the chick embryo. I also study the resetting of the epigenome in primordial germ cells as well as during cellular reprogramming. In parallel, I participate in the generation of a virtual stem cell model ...
| Guillaume Montillet || |
Selected Publications of Team members : |
Kress C, Montillet G, Jean C, Fuet A, Pain B (2016) Chicken embryonic stem cells and primordial germ cells display different heterochromatic histone marks than their mammalian counterparts 2016 Feb 10;9:5. doi: 10.1186/s13072-016-0056-6
Bachelard E, Raucci F, Montillet G, Pain B (2015) Identification of side population cells in chicken embryonic gonads Theriogenology. 2015 Feb;83(3):377-84
Raucci F, Fuet A, Pain B (2015) In vitro generation and characterization of chicken long-term germ cells from different embryonic origins Theriogenology. 2015 Sep 15;84(5):732-742.e2
Chen H, Aksoy I, Gonnot F, Osteil P, Aubry M, Hamela C, Rognard C, Hochard A, Voisin S, Fontaine E, Mure M, Afanassieff M, Cleroux E, Guibert S, Chen J, Vallot C, Acloque H, Genthon C, Donnadieu C, De Vos J, Sanlaville D, Guérin JF, Weber M, Stanton LW, R (2015) Reinforcement of STAT3 activity reprogrammes human embryonic stem cells to naive-like pluripotency Nat Commun. 2015 May 13;6:7095
Couteaudier M, Trapp-Fragnet L, Auger N, Courvoisier K, Pain B, Denesvre C, Vautherot JF (2015) Derivation of keratinocytes from chicken embryonic stem cells: establishment and characterization of differentiated proliferative cell populations Stem Cell Res. 2015 Mar;14(2):224-37
Jean C, Oliveira NM, Intarapat S, Fuet A, Mazoyer C, De Almeida I, Trevers K, Boast S, Aubel P, Bertocchini F, Stern CD, Pain B (2015) Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC Stem Cell Res. 2015 Jan;14(1):54-67
Bouhallier F, Allioli N, Lavial F, Chalmel F, Perrard MH, Durand P, Samarut J, Pain B, Rouault JP. (2010) Role of miR-34c microRNA in the late steps of spermatogenesis. RNA. 16(4): 720-31 download
Lavial F, Pain B (2010) Chicken embryonic stem cells as a non-mammalian embryonic stem cell model Dev Growth Differ. 52(1): 101-14 download
Lavial F, Acloque H, Bachelard E, Nieto MA, Samarut J, Pain B. (2009) Ectopic expression of Cvh (Chicken Vasa homologue) mediates the reprogramming of chicken embryonic stem cells to a germ cell fate. Dev Biol. 330(1): 73-82. download
Lavial F, Acloque H, Bertocchini F, Macleod DJ, Boast S, Bachelard E, Montillet G, Thenot S, Sang HM, Stern CD, Samarut J, Pain B (2007) The Oct4 homologue PouV and Nanog regulate pluripotency in chicken embryonic stem cells. Dev 134(19):3549-3563 download