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Conference by Adrien Meguerditchian

Published on March 28, 2017



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 Teams 

Postnatal forebrain development and plasticity

 

The mechanisms that regulate the generation of neuronal diversity in the developing central nervous system (CNS) have been extensively studied. These studies have shown that both the spatial and temporal origins of precursors determine their neurotransmitter phenotype, firing properties, subtype specification and the position of the cell body in different regions and different layers.


These rules seem to persist into adulthood in the sub-ventricular zone (SVZ) of the lateral ventricle, i.e. the main adult germinal zone of the adult forebrain.


As observed in the embryo, neural stem cells (NSCs) in distinct location of the lateral ventricle contribute to the generation of neurons that will migrate to different layers of the olfactory bulb and express distinct subtype specific markers.


These observations suggest that rather than being plastic and homogeneous, neural stem cells of the lateral ventricle are a restricted and diverse population of progenitors.  
By using a combination of molecular and imaging tools, our research addresses the following aims:
•    To better understand the diversity of neural stem cells lineages in the postnatal forebrain
•    To identify the transcription factors and signaling pathways involved in neuronal differentiation and lineage specification
•    To manipulate expression of these transcription factors in pathologies of the central nervous system in order to promote repair

You can also find relevant information about this team here.



Dr Olivier Raineteau

Diane Angonin

Diane combines fate mapping techniques with targeted lesions of defined olfactory bulb neuronal subtypes, in order to test the regenerative potential of early postnatal subventricular zone progenitor populations. She is also interested in testing the effects of canonical Wnt stimulation to promote dorsal progenitor fates (oligodendroglial and glutamatergic) in health and diseases.


Vanessa Donega

During my PhD I investigated the potential of intranasal mesenchymal stem cell administration to promote regeneration of the postnatal brain following a hypoxic-ischemic insult. I joined the lab as a Post-Doctoral researcher to study the response of defined progenitor populations to neonatal hypoxia, and if their participation to tissue repair can be encouraged by pharmacological stimulation of signaling pathways.

 


Elodie Gaborieau

Elodie focuses on the molecular control of progenitors proliferation in the postnatal subventricular zone. A long term aim of her work consists in understanding the relations that may exist between progenitor’s proliferative behavior and acquisition of defined fates.


Guillaume Marcy PhD Student

Guillaume’s work aims at addressing postnatal subventricular zone progenitor’s competence, i.e. their capacity to generate a diversity of cell type. To this end, he is using two approaches: 1) heterotypic/heterochronic transplantations; 2) targeted ablations of defined neuronal subtypes.


Stefan Zweifel

Stefan’s work aims at identifying transcription factors that actively participate into the regionalization of the postnatal subventricular zone. He is currently focusing on transcription factors that are enriched in the dorsal most microdomains of this germinal region, where they may control neuronal vs. glial fate choice.


Selected Publications of Team members :
Orlando C, Raineteau O (2015) Integrity of cortical perineuronal nets influences corticospinal tract plasticity after spinal cord injury Brain Struct Funct. 2015 Mar;220(2):1077-91
Azim K, Hurtado-Chong A, Fischer B, Kumar N, Zweifel S, Taylor V, Raineteau O. (2015) Transcriptional Hallmarks of Heterogeneous Neural Stem Cell Niches of the Subventricular Zone Stem Cells. 2015 Jul;33(7):2232-42
Amrein I, Nosswitz M, Slomianka L, van Dijk RM, Engler S, Klaus F, Raineteau O, Azim K (2015) Septo-temporal distribution and lineage progression of hippocampal neurogenesis in a primate (Callithrix jacchus) in comparison to mice Front Neuroanat. 2015 Jun 29;9:85. doi: 10.3389/fnana.2015.00085. eCollection 2015
Azim K, Fischer B, Hurtado-Chong A, Draganova K, Cantu C, Zemke M, Sommer L, Butt A, Raineteau O (2014) Persistent Wnt/b-Catenin signaling determines dorsalization of the postnatal subventricular zone and neural stem cell specification into oligodendrocytes and glutamatergic neurons Stem Cells, 32(5):1301-12
Fischer B, Azim K, Hurtado-Chong A, Ramelli S, Fernández M, Raineteau O. (2014) E-proteins orchestrate the progression of neural stem cell differentiation in the postnatal forebrain. Neural Dev. 9(1):23