CORTEX International Meeting 2013Published on April 22, 2013
LabEx CORTEX is organising an International Conference in Lyon next June....
Embryonic Stem Cells: Self-renewal and Early Commitment
Our team examines the mechanisms of self-renewal and pluripotency in mouse and human embryonic stem cells. We aim to identify novel genes involved in the maintenance of the undifferentiated state and in early commitment towards ectodermal, mesodermal and endodermal lineages. Another aspect of our work focuses on the regulation of the cell cycle, which shows unusual characteristics in pluripotent stem cells. We explore the role of regulators of the G1- to S-phase transition in the maintenance of the undifferentiated state. We also make use of the peptide aptamer technology to create novel recombinant proteins supporting self-renewal of human ES cells.
| Pierre Savatier || |
| Pierre-Yves Bourillot || |
Investigating the mechanisms of STAT3-dependant self-renewal, the hallmark of mouse ES cells, and understanding why this pathway does not operate in primate, human and non human ES cells.
| Hongwei Chen || |
----Investigating the capacity of rhesus ES cells to colonize the monkey blastocyst. ----Exploring STAT3 signaling in rhesus and human ES cells.
| Diana Coronado || |
Investigating the function of cyclin E and cycin E-associated kinase activity in self-renewal and pluripotency in mouse and human ES cells
| Vincent Giudice || |
Investigating the function of novel STAT3 target genes in the maintenance of mouse and human ES cells in the undifferentiated state
| Yann Tapponnier || |
Selected Publications of Team members : |
Bourillot PY, Aksoy I, Schreiber V, Wianny F, Schulz H, Hummel O, Hubner N, Savatier P. (2009) Novel STAT3 target genes exert distinct roles in the inhibition of mesoderm and endoderm differentiation in cooperation with nanog Stem Cell 27(8): 1760-1771 download
Schulz H et al (2009) The FunGenES database: a genomic resource for mouse Embryonic Stem Cells differentiation PloS One, 4(9): e6804. download
Marchand M, Schröder I, Markossian S, Skoudy A. Nègre D. Cosset FL, Real FX, Wobus AM, Savatier P (2009) Mouse ES cells overexpressing the transcription factor NeuroD1 show increased differentiation towards endocrine lineages Int. J. Dev. Biol. 53:569-578. download
Wianny F, Bernat A, Huissoud C, Marcy G, Markossian S, Cortay V, Giroud P, Leviel V, Kennedy H, Savatier P, Dehay C (2008) Derivation and cloning of a Novel Rhesus ES cell line stably expressing Tau-GFP Stem Cells 26(6):1444-53 download
Aksoy I, Sakabedoyan C, Bourillot PY, Malashicheva AB, Mancip J, Knoblauch K, Afanassieff M, Savatier P (2007) Self-renewal of murine embryonic stem cells is supported by the serine/threonine kinases pim-1 and pim-3. Stem Cells 25(12):2996-3004 download
Fluckiger AC, Marcy G, Marchand M, Negre D, Cosset FL, Mitalipov S, Wolf D, Savatier P, Dehay C (2006) Cell cycle features of Primate Embryonic Stem Cells Stem Cells 24(3):547-556 download
Lukaszewicz A, Savatier P, Cortay V, Giroud P, Huissoud C, Berland M, Kennedy H, Dehay C (2005) G1 phase regulation, Area-Specific cell cycle control, and Cytoarchitectonics in the Primate Cortex Neuron 47 (3):353-64 download
Jirmanova L, Afanassieff M, Gobert-Gosse S, Markossian S, Savatier P (2002) Differential contributions of ERK and PI3-kinase to the regulation of cyclin D1 expression and to the control of the G1/S transition in mouse embryonic stem cells. Oncogene 21:5515-28 download
Burdon T, Smith AG, Savatier P (2002) Signalling, cell cycle and pluripotency in embryonic stem cells. Trends Cell Biol 12:432-8 download
Bourillot PY, Garrett N, Gurdon JB (2002) A changing morphogen gradient is interpreted by continuous transduction flow. Development 129:2167-2180. download
Vallier L, Mancip J, Markossian S, Lukaszewicz A, Dehay C, Metzger D, Chambon P, Samarut J, Savatier P (2001) An efficient system for conditional gene expression in embryonic stem cells and their in vitro and in vivo differentiated derivatives. Proc Nat Acad Sci USA 98:2467-2472 download
Gurdon JB, Bourillot PY (2001) Morphogen gradient interpretation. Nature 413:797-803. download
Savatier P, Lapillonne H, van Grunsven LA, Rudkin BB, Samarut J (1996) Withdrawal of differentiation inhibitory activity/leukemia inhibitory factor up-regulates D-type cyclins and cyclin-dependent kinase inhibitors in mouse embryonic stem cells. Oncogene 12:309-322 download