Howard Cooper Team Leader / DRE
The research aims of Neurobiological Rhythms and Sleep are focused on the molecular, cellular and behavioral mechanisms of the circadian timing system and the consequences of aging and neurodegenerative disease. Central to our approach is an understanding of the synchronization of circadian rhythms by the external light cycle, the molecular and physiological mechanisms of the endogenous circadian oscillator (SCN), and the regulation of output behavioral and physiological rhythms.
The coding of photic information by retinal photoreceptors (rods, cones, melanopsin ganglion cells) are studied using in vivo electrophysiological recording techniques in anaesthetised and awake, freely moving animals. The effects of light (intensity, duration, spectrum) on SCN neuronal activity and on clock gene expression are also assayed using quantitative RT-PCR, laser dissection and microarray analysis.
In order to understand the consequences of chronobiological disorders, another line of research involves investigation of the mechanisms of synchronisation of central and peripheral oscillators, including the expression of clock genes and rhythmically expressed clock controlled genes in the brain and in different body tissues.
Pathological models investigated in rodents include ocular diseases and Parkinson's disease, and a prosimian primate model of aging. In humans, circadian photoreception and entrainment of the circadian timing system as well as chronobiological disorders related to ocular pathologies, aging and neurodegenerative diseases are studied in the framework of a European integrated project in our clinically based Platform for Research on Human Chronobiology. In order to bridge the gap between cellular-molecular studies in rodent models and clinical studies in humans, the non-human primate is used to study the circadian timing system and sleep wake cycle and, in the framework of the laboratory transversal project, the chronobiological consequences of Parkinson's Disease.
|2017||3:9||Global rise of potential health hazards caused by blue light-induced circadian disruption in modern aging societies||Hatori M, Gronfier C, Van Gelder RN, Bernstein PS, Carreras J, Panda S, Marks F, Sliney D, Hunt CE, Hirota T, Furukawa T, Tsubota K||NPJ Aging Mech Dis|
|2016||57(3):1063-71||Heterochromatic Flicker Photometry for Objective Lens Density Quantification||Najjar RP, Teikari P, Cornut PL, Knoblauch K, Cooper HM, Gronfier C||Invest Ophthalmol Vis Sci||-|
|2016||9:509||The Role of Astroglia in the Antidepressant Action of Deep Brain Stimulation||Etiévant A, Lucas G, Dkhissi-Benyahya O, Haddjeri N||Front Cell Neurosci||-|
|2015||47:24-34||Influence of sleep deprivation and circadian misalignment on cortisol, inflammatory markers, and cytokine balance||Wright KP Jr, Drake AL, Frey DJ, Fleshner M, Desouza CA, Gronfier C, Czeisler CA||Brain Behav Immun||-|
|2015||131:136-42||A 5-HT3 receptor antagonist potentiates the behavioral, neurochemical and electrophysiological actions of an SSRI antidepressant||Bétry C, Overstreet D, Haddjeri N, Pehrson AL, Bundgaard C, Sanchez C, Mørk A||Pharmacol Biochem Behav||-|