SBRI Stem-cell and Brain Research Institute - France (Lyon)

Technical Facilities : Molecular Biology Facility

The unit has developed a facility, fully equipped for molecular biology techniques. All these different pieces of equipment are managed by the person listed below. As well as managing the equipment, these persons carry out training programs for junior researchers in the various technologies.

Composition of team / September 1, 2017

Coordinator : Pierre Yves Bourillot

  • Camille Baquere - Engineer (AI)
  • Barabara Berarducci - Engineer (AI)
  • Thierry Blachere - Engineer (IR)
  • Pierre Yves Bourillot - Researcher
  • Christine Coutanson - Engineer (AI)
  • Nathalie Doerflinger - Engineer (IR)
  • Aurélie Fuet - Engineer (IE)
  • Christian Jean - Engineer (AI)
  • Guillaume Marcy - Engineer (IE)
  • Guillaume Montillet - Engineer (AI)
  • Cloe Rognard - Engineer (IE)

Description Equipment

NUCLEIC ACID ANALYSIS

  • Nucleic Acid Quantitation and Quality Control
    • BioPhotometer (Eppendorf)
    • NanoDrop Spectrophotometer (Life’s)
    • Qubit 2.0 Fluorometer (Invitrogen)
  • PCR Machines
    • T3 Thermocycler (Biometra)
    • T3000 Thermocycler (Biometra)
    • PTC-200 (MJ Research)
    • Gene Touch (Bio Concept)
  • Real Time PCR Machines
    • LightCycler 1.5 (Roche)
    • StepOnePlus (Applied BioSystems) (x2)
    • Mx3000P (Stratagene)
  • PCR Workstation
    • Workstation 36”wide (CBS Scientific)
    • Standard UV PCR Systems (UVP)
  • Gel Electrophoresis Systems
  • Digital Imaging System
    • Mega Bio-Print (Biotech)

PROTEIN ANALYSIS

  • PAGE and Western Blotting Apparatus
    • Mini Protean System (Bio-Rad)
    • Protean II xi Cell (Bio-Rad)
    • Trans-Blot Turbo Transfer System (Bio-Rad)
    • SNAP i.d. Protein Detection System (Millipore)
  • Digital Imaging System
    • ChemiDoc MP Imaging system (Bio-Rad)

PROTEIN PURIFICATION

  • HPLC
    • Ultimate 3000 Pump (Dionex)
    • Spectra System AS3500 Injector (Thermo Separation Products)
    • Coulochem III Electrochemical Detector (Dionex)

MICROPLATE READERS

  • Luminometer
    • Glomax Multi Detection System (Promega)

BACTERIA CULTURE (L1 SAFETY LAB)

  • Incubator
    • Innova 4080 Shaker (New Brunswick)
    • C25 Incubator Shaker (New Brunswick)
    • Heratherm Advanced Protocol Microbiological Incubator (ThermoScientific)
  • Class II Biosafety Cabinet
    • MSC Advantage ((ThermoScientific)
  • Centrifuge
    • Avanti J-E High Speed (Beckman Coulter)

CELL ANALYSER

  • LSR Fortessa X20 4 lasers (BD Biosciences)

ULTRASONICATOR

  • Bioruptor Plus (Diagenode)

LASER CAPTURE MICRODISSECTION

The microscope is an upright Leica DM6000 B.

Microscope with motorized objective nosepiece:

  • One objective for the overview:
    • 1.25x/0.04 HCX PL FLUOTAR
  • Five objectives for microdissection:
    • 5x/0.12 UVI Microdissection
    • 10x/0.30 HC PL FLUOTAR
    • 20x/0.40 HCX PL FL L CORR PH1
    • 40x/0.60 HCX PL FL L CORR PH2
    • 63x/0.70 HCX PL FL L CORR XT
  • Camera Leica DFC 310FX
  • Camera LMD CC7000 digital color
  • Fluorescence source EL6000 with three filters:
    • LMD-DAPI
    • LMD-GFP
    • LMD-RFP
  • UV laser for microdissection
  • Motorized stage with a three slides holder
  • Two collectors for tube caps:
    • 4x 0.2ml PCR tubes
    • 4x 0.5ml PCR tubes
  • Leica LMD Software

GENERAL EQUIPEMENT

  • Hybridisation Ovens
    • UNB100 (Memmert)
    • UNB200 (Memmert)
    • UFE400 (Memmert)
    • Heratherm (Thermoscientific)
    • HybEZ Oven (ACD Advanced Cell Diagnostics)
  • -80°C Freezer
    • HFU T Series (Thermoscientific) (x2)
    • HERA FREEZE BASIC (Thermoscientific)
    • VIP SERIES (Sanyo) (x2)
    • HEF U570 (New Brunswick)
    • Innova U535 (New Brunswick)
    • -80°C FREEZER (FormaScientific)
    • HERAfreeze (Heraeus)

DATA ANALYSIS SOFTWARE

  • FlowJo (FlowJo)
  • BD FACSDIVATM SOFTWARE (BD Biosciences)