cells (ESC, EpiSC or iPSC) from different species are routinely cultured in vitro and represent a tremendous hope
for numerous therapies. However, derivation of ES cells and production of iPS
cells are very inefficient in non-rodent species; the quality of these cells
remains variable; and only rodent ES and iPS cells maintain the most naive
pluripotency state similar to the inner cell mass.
The objectives of my research
are then to improve the derivation and purification of safe pluripotent cells. For
these purposes, my current projects focus on:
- understanding the
mechanisms involved in the maintenance of naive or primed states by focusing on
the retroviral silencing in mouse, as a model, in chicken (non-mammalian) and
rabbit (mammalian) as two non-rodent models.
- defining novel
biomarkers to distinguish pluripotency states.
- developing new tools like reporter genes to mark
and/or select pluripotent stem cells.